Cancer Drug Resistance: Methods and Protocols

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Cancer Drug Resistance: Methods and Protocols

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Details advanced methodologies for investigating why tumors evade medical treatment. The text explores computational workflows and polygenomic analysis to integrate data from DNA methylation, RNA expression, and single nucleotide polymorphisms. Laboratory techniques are extensively described, including mass cytometry for analyzing cell heterogeneity, ATAC-seq for mapping epigenetic changes, and microfluidic devices for modeling the tumor microenvironment. Researchers also provide specific procedures for using metabolic modeling to predict drug targets and investigating ER-phagy as a cellular survival mechanism. These resources aim to provide a comprehensive toolkit for identifying biomarkers and developing personalized therapeutic strategies to overcome therapy evasion.

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