AAV Vectors For In Vitro and In Vivo Uses
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AAV Vectors For In Vitro and In Vivo Uses
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When combined with modern technology for gene expression including cells-specific promoters as well as the Cre/lox system and genome-editing, AAVs provide a feasible quick, cost-effective, and affordable alternative to conditional knockouts or the transgenic mice models. But, there are many obstacles to widespread AAV cell line use, including ineffective methods for purification and small viral numbers. We present an improved procedure to make AAVs with serotypes that are not dependent on their genotypes.
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